Considerações sobre biosegurança em arquitetura de biotérios (Telma Abdalla de Oliveira Cardoso) (in Portuguese)
Abstract: Biosafety considerations in the architecture of laboratory-animal housing
This work seeks the reflection of the development of the Brazilian scientific research, relative to the architectural programming of laboratory-animal housing and of laboratories of animal experimentation, tends towards the premise that design criteria is an important aspect that contributes to the reliability of the experimental results. For the discussion of the variables that characterize the science of laboratory animals, the diversity of the team involved, needs guidelines on which to base the process of planning the constructions. Architectural programming represents one in the ways of thinking for one's own research activity, intending to define the appropriate space and design conditions so that the researcher’s work can be developed.
Imnmunohistochemical recognition of a wide spectrum of lyssaviruses in formalin-fixed tissues by one monoclonal antibody
(Jorge W. López, Charles V. Trimarchi, Alexander I. Wandeler, Yvonne van Gessel) (en inglés)
Abstract: Automated indirect immunoperoxidase (avidin-biotin complex) staining using monoclonal antibody #5DF12-3B6, directed against rabies N-protein, was used to detect rabies antigen in tissue samples from animals either naturally or experimentally infected with rabies. This monoclonal antibody recognized all 16 strains of rabies virus tested, as well as rabies-related lyssaviruses including Duvenhage, Lagos Bat, and Mokola. The sample infected with Mokola virus initially showed only weak staining, however, deletion of protease digestion resulted in stronger stain uptake. The test was sensitive and specific, correctly identifying rabies antigen in all but one of the samples tested (37/38), and no apparent staining in any of the negative samples tested (23/23). Tissues from 16 mammalian species were tested, including one rabies infected human tissue sample. The utility of the immunoperoxidase staining method described in this study lies in the ability of one monoclonal to recognize a broad spectrum of lyssaviruses in formalin-fixed tissues.
Vacinação emergencial de bovinos contra a febre aftosa (Gilfredo Comparsi Darsie, José Lourenço dos Reis, Alberto Knust Ramalho) (in Portuguese)
Abstract: Emergency vaccination for foot-and-mouth disease in cattle
The use of a monovalent vaccine containing strain O1 Campos antigen with the adjuvant saponin was tested for emergency use in areas free of foot-and-mouth disease. Certain favorable results support its use.
Use of a recombinant foot-and-mouth disease virus 3D polymerase in an agarose gel immunodiffusion test (Bergman Ingrid E., Malirat Viviana, Falczuk Abraham, Söndahl Magnus S.)
Abstract: In the present study, examination was made of the effectiveness of a complete recombinant 3D polypeptide in an AGID test (AGID-3D), for use in the detection of FMDV-infection-specific antibodies, regardless of vaccination condition. Results indicate that compared with the traditional AGID VIAA, the AGID 3D offers, particularly when assessing low titer sera, a more consistent method, with comparable specificity, and at least equal sensitivity. Neither of the antigens offered any particular advantage with regard to band differentiation. Replacement of the VIAA by a recombinant 3D antigen has considerable attractions, since it provides an unlimited supply of a safe, inexpensive, easily purified and consistent material, eliminating the potential presence of non-specific BHK or capsideal antigens. español
Abstracts: List | Chapter containing all abstracts
- Baranowski, E. et al., Multiple virulence determinants of foot-and-mouth disease virus in cell culture.
- Cameron, A.R. & F.C. Baldock: A new probability formula for surveys to substantiate freedom from disease.
- Clercq, K., Implementation of quality assurance in national foot-and-mouth disease laboratories, based on the guidelines of the Office International des Épizooties.
- Cox, S.J. et al., Emergency vaccination of sheep against foot-and-mouth disease: protection against disease and reduction in contact transmission.
- Dufour, B., Technical and economic evaluation method for use in improving infectious animal disease surveillance networks
- Hutber, A.M. et al., Control of foot-and-mouth disease through vaccination and the isolation of infected animals.
- López Inzaurralde, A. & E.C. Moreira, Vesicular stomatitis epidemiology in Brazil: a review.
- MacKay, D.K.J. et al., Differentiating infection from vaccination in foot-and-mouth disease using a panel of recombinant, non-structural proteins in ELISA.
- Mateu, M.G. et al., Mutational analysis of discontinuous epitopes of foot-and-mouth disease virus using an unprocessed capsid promoter precursor.
- Mattion, N. et al., Emergency foot-and-mouth disease vaccine: early induction of immunity in susceptible species.
- Murphy, M.L.P. et al., Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues
- Niedbalski, W. et al., Quantification of foot-and-mouth disease virus genomes in bovine tissue by competitive RT-PCR.
- Ried, S.M. et al., Comparison of reverse transcription polymerase chain reaction, enzyme linked immunosorbent assay and virus isolation for the routine diagnosis of foot-and-mouth disease.
- Reid, S.M. et al., Diagnosis of foot-and-mouth disease by RT-PCR: evaluation of primers for serotypic characterization of viral RNA in clinical samples.
- Sadir, A.M. et al., Improvement of the immune response to foot-and-mouth disease vaccine in calves by using Avridine as adjuvant.
- Salt, J.S. et al., Emergency vaccination of pigs against foot-and-mouth disease: protection against disease and reduction in contact transmission.
- Sanmartino, L.E. et al., Evaluation of the protection against brucellosis provided by strain 19 in cattle vaccinated simultaneously wiht oil-adjuvanted foot-and-mouth disease vaccine.
- Saraiva, V., Methodological bases for the characterization of risk.
- Sevilla, N. et al., An RNA virus can adapt to the multiplicity of infection.
- Shen, F. et al., Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA
- Sorensen, K.J. et al., Differentiation of infection from vaccination in foot-and-mouth disease by the detection of antibodies to the non-structural proteins 3D, 3AB and 3ABC in ELISA using antigens expressed in baculovirus.
- Toja, M. et al., Genomic nucleotide sequence of a foot-and-mouth disease virus clone and its persistent derivatives. Implications for the evolutionb of viral quasispecies during a persistent infection.